克隆性分析技术在肝脏局灶性结节性增生诊断中的应用
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巩 丽, 张宏图, 张 伟, 李爱宁, 刘 杉,
克隆性;局灶性结节性增生;诊断,巩丽,张伟,李爱宁,刘贵秋,苏勤,张宏图,刘杉,孙耘田,苏勤,巩丽,通讯作者:,Applicationofclo
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巩丽, 张伟, 李爱宁, 刘贵秋, 苏勤, 中国人民解放军第四军医大学唐都医院病理科 陕西省西安市 710038
张宏图, 刘杉, 孙耘田, 苏勤, 中国医学科学院中国协和医科大学肿瘤医院病理科 北京市 100021
巩丽, 2003年级第四军医大学唐都医院病理科硕士生.
国家自然科学基金资助项目, No. 30171052和No. 30572125
通讯作者: 苏勤, 100021, 北京市潘家园南里17号, 中国医学科学院中国协和医科大学肿瘤医院病理科. q.su@wjgnet.com
电话: 010-87788435 传真: 010-67713359
收稿日期: 2005-12-27 接受日期: 2006-01-13
Application of clonality analysis in diagnosis of focal nodular hyperplasia
Li Gong, Hong-Tu Zhang, Wei Zhang, Ai-Ning Li, Shan Liu, Yun-Tian Sun, Gui-Qiu Liu, Qin Su
Li Gong, Wei Zhang, Ai-Ning Li, Gui-Qiu Liu, Qin Su, Department of Pathology, Tangdu Hospital, the Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China
Hong-Tu Zhang, Shan Liu, Yun-Tian Sun, Qin Su, Department of Pathology, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China
Supported by National Natural Science Foundation of China, No. 30171052 and No. 30572125
Correspondence to: Dr. Qin Su, Department of Pathology, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 17 Panjiayuan Nanli, Beijing 100021, China. q.su@wjgnet.com
Received: 2005-12-27 Accepted: 2006-01-13
Abstract
AIM: To study the clonality status of the whole lesions and each nodules of hepatic focal nodu-lar hyper-plasia (FNH), and simultaneously compare the clonal composition with hepato-cellular adenoma (HA).
METHODS: Four FNHs from 3 women, 2 HAs and 4 HCCs were examined by clonality assays based on X-chromosome inactivation mosaicism. Nodules were microdissected from paraffin sections. Genomic DNA was isolated from each nodules, the whole lesions and surrounding liver parenchyma, pretreated with Hpa II or Hha I, and then amplified via nested polymerase chain reaction (PCR) for phosphoglycerate kinase (PGK) and androgen receptor (AR) genes ......
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