HCV p7蛋白反式调节基因p7TP2的克隆化及生物信息学分析
http://www.100md.com
袁 菊, 郭 江, 成 军, 陶明亮, 蓝贤勇,
HCVp7蛋白;反式调节基因;克隆化;生物信息学分析,HCVp7蛋白反式调节基因p7TP2的克隆化及生物信息学分析,袁菊,郭江,成军,陶明亮,蓝贤勇,洪源,毛羽,袁菊,陶明亮,蓝贤勇,靳亚平,袁菊
 |
| 第7页 |
 |
| 第1页 |
参见附件(976KB，7页)。
袁菊, 郭江, 成军, 陶明亮, 蓝贤勇, 洪源, 毛羽, 北京地坛医院传染病研究所 北京市 100011
袁菊, 陶明亮, 蓝贤勇, 靳亚平, 西北农林科技大学动物科技学院 陕西省杨陵区 712100
袁菊, 西北农林科技大学2004级硕士生, 主要研究方向动物生殖内分泌学.
国家自然科学基金资助项目, No. 30371288
国家重点基础研究973项目, No. 2004CB518908
通讯作者: 成军, 100011, 北京市东城区安外大街地坛公园13号, 北京地坛医院传染病研究所. cj@genetherapy.com.cn
电话: 010-64481639 传真: 010-64281540
收稿日期: 2005-12-09 接受日期: 2006-01-14
Cloning and bioinformatics analysis of p7TP2 gene transregulated by hepatitis C virus p7 protein
Ju Yuan, Jiang Guo, Jun Cheng, Ming-Liang Tao, Xian-Yong Lan, Yuan Hong, Ya-Ping Jin, Yu Mao
Ju Yuan, Jiang Guo, Jun Cheng, Ming-Liang Tao, Xian-Yong Lan, Yuan Hong, Yu Mao, Institute of Infectious Diseases, Beijing Ditan Hospital, Beijing 100011, China
Ju Yuan, Ming-Liang Tao, Xian-Yong Lan, Ya-Ping Jin, College of Animal Science and Technology, Northwest A & F University, Yang Ling 712100, Shaanxi Province, China
Supported by National Natural Science Foundation of China, No. 30371288, and the Special Funds for Major State Basic Research 973 Program of China, No. 2004CB518908
Correspondence to: Dr. Jun Cheng, Institute of Infectious Diseases, Beijing Ditan Hospital, Dongcheng District, Anwai Avenue, 13 Ditan Park, Beijing 100011, China. cj@genetherapy.com.cn
Received: 2005-12-09 Accepted: 2006-01-14
Abstract
AIM: To clone and identify a new gene p7TP2 transregulated by hepatitis C virus p7 protein, and construct its recombinant eukaryotic expression vector to explore its function and structure.
METHODS: p7TP2 gene was amplified by reverse chain polymerase chain reaction (RT-PCR) using HepG2 cDNA as template and inserted into the vector pGEM-T by TA cloning. Recombinant eukaryotic expression vector (pcDNATM3.1/myc-His A-p7TP2) was then constructed by subcloning technique which were all confirmed by restriction enzyme digestion analysis and sequencing ......
您现在查看是摘要介绍页，详见PDF附件(976KB，7页)。