关键词: 基因，DCC;串联重复序列;动脉粥样硬化;遗传易感性

    摘 要:目的 探讨DCC基因STR位点多态性与动脉粥样硬化遗传易感性之间的关系. 方法 利用PCR-STR方法对陕西正常老年人群(52例)动脉粥样硬化(57例)的DCC基因STR位点多态性进行群体遗传学研究，并就该位点与动脉粥样硬化关系进行关联分析. 结果 对照组陕西正常老年人群DCC基因STR位点，扩增片断基因频率分布0.0096～0.3270，片断重复次数为37～92;DCC基因STR位点，在正常老年人群中多态信息量(PIC)为0.754，杂合度(H)为0.812.结果对比显示，病例组与对照组等位基因频率分布有显著差异(χ2 =38.792，df=13，P<0.01)，其中动脉粥样硬化组250～270bp范围内基因频率高于对照组(P<0.01)，对照组180～190bp范围内基因频率高于动脉粥样硬化组(P<0.01);病例组与对照组杂合度与多态性分析，未见显著性差异. 结论 DCC基因STR位点多态性改变可能与动脉粥样硬化遗传易感性相关联，等位基因片断长度在250～270bp范围内的可能是动脉粥样硬化的遗传标记.

    Study on genetic polymorphisms of DCC gene STR in atherosclerosis

    YANG Yong SHU Qing ZHENG Qiang-Sun LI Jun-Ling FENG Lei

    1 Department of Genetics and Growth Biology，Fac-ulty of Preclinical Medicine，Fourth Military Medi-cal University，Xi'an710033，China，

    2 Department of Cardiovasology，Tangdu Hospital，Fourth Mili-tary Medical University，Xi'an710038，China

    Keywords:genes，DCC;tandem repeat sequences;atherosclerosis;susceptibility

    Abstract:AIM To study the relationship between genetic polymorphisms of DCC gene STR in Shaanxi people and its possible association with the susceptibility of atherosclerosis.METHODS Polymorphisms of DCC gene STR was studied by PCR-STR in52unrelated individuals and57atheroscle-rosis samples in Shaanxi people.RESULTS The frequency of alleles which were amplified in the control group was from0.0096～0.3270and the number of repetition was from37to92.Polymorphism information content(PIC)of DCC gene STR was0.754and the heterozygosity(H)was0.812.The distribution of allele frequency between atherosclerosis and control group was significantly different(χ2 =38.792 ......