关键词: 肝炎病毒，乙型;基因型;聚合酶链反应;多态性，限制性片段长度

    摘 要:目的 了解陕西地区乙肝病毒(HBV)基因分型情况. 方法 用套式PCR方法从100份HBsAg阳性的乙型肝炎患者血清中扩增585bp的S基因，用限制性内切酶BsrⅠ，StyⅠ，DpnⅠ和HpaⅡ平行酶切鉴别HBV A～F基因型. 结果 所有标本用内引物P1和P2均扩增获得的585bp的靶基因，继之用上述限制性内切酶消化鉴定，证明100份血清标本中的HBV可分为B型36份(36%)，C型56份(56%)，D型8份(8%). 结论 陕西地区HBV分型以B，C型为主，D型较少，未发现A，E，F型.结果同时表明PCR-RFLP方法灵敏性高，特异性强，且酶切图谱简明单一，易于识别，适用于HBV基因型的流行病学调查和临床实验研究.

    Genotyping of Hepatitis B viruses from hepatitis B patients in Shaanxi province

    ZHANG Yan，BAI Xue-Fan，LI Xin-Hong，LI Yu，XIE Yu-Mei，KANG Wen-Zhen，ZHAO Yu-Ling

    Center of Infectious Diseases of Chinese PLA，Tangdu Hospital，Fourth Military Medical Univer-sity，Xi'an710038，China

    Keywords:hepatitis B virus;genotype;polymerase chain re-action;polymorphism，restriction fragment length

    Abstract:AIM To investigate the genotypes of Hepatitis B virus(HBV)in Shaanxi province.METHODS The585bp length of HBV S gene was amplified from the blood samples of100patients with HBsAg positive using polymerase chain reaction(PCR).The PCR products were digested with four kinds of restriction enzymes ......