关键词: SCID小鼠;免疫重建;肝癌细胞;免疫细胞

    摘 要:目的 探讨SCID小鼠免疫重建及免疫对人肝癌细胞成瘤的影响. 方法 体外培养人肝癌细胞系，接种到SCID小鼠和用BALB/c，CBA/N小鼠外周血淋巴细胞重建的SCID(B-PBL-SCID和C-PBL-SCID)小鼠皮下;监测成瘤状态，测定鼠脾细胞毒杀伤力和免疫重建的免疫球蛋白量，作血清与靶细胞凝集实验. 结果 免疫重建体现了所用小鼠免疫对肿瘤的排斥特点，免疫重建成功;SCID和C-PBL-SCID小鼠100%成瘤(两瘤体相比P<0.01)，B-PBL-SCID出现瘤体后逐渐消失;B-PBL-SCID和C-PBL-SCID脾细胞对靶细胞有一定的杀伤力，B-PBL-SCID的血清能与靶细胞产生凝集反应. 结论 SCID是建立肿瘤模型和肿瘤免疫研究的良好动物;肝癌细胞在小鼠体内的成瘤率与T，B细胞免疫有关.

    Characteristics of the human hepatocarcinoma cells growth in SCID mice and immunoreconstituted SCID mice

    ZHANG Zhi-Pei JIANG Xun HUANG Li-Jun ZHAO Zuo-Qing SHI Xin-You LI Liu-Jin SHI Chang-Hong

    1 Department of Experimental Surgery，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China，

    2 Center of Experimental Ani-mals，Fourth Military Medical University

    Keywords:SCID mice;immunoreconstitution;hepatocarci-noma cell;immune cell

    Abstract:AIM To explore the immunoreconstitution of SCID mice and the affection of immunity on hepatocarcinoma cell growth in vivo.METHODS The human hepatocarci-noma cells cultured in vitro were inoculated into the limbs of SCID mice subcutaneously and the immunoreconstituted SCID mice with BALB/c and CBA/N mice peripheral blood lymphocytes(B-PBL-SCID and C-PBL-SCID mice)and their growth situations were monitored.The spleen cells of these mice were harvested in5weeks after inoculation and exam-ined for direct cytotoxicity to the hepatocarcinoma cells(tar-get cells).The immunoglobulin concentration of B-PBL-SCID mice was surveyed and the serum agglutination of B-PBL-SCID mice was tested.RESULTS The successfully re-constituted immunity in SCID mice embodied the rejection characteristics of BALB/c and CBA/N mice to the hepatocar-cinoma cells.There was100%carcinoma growth in SCID and C-PBL-SCID mice ......