关键词: 汉滩病毒;84FLi株;序列分析;系统发生树

    摘 要:目的 测定我国肾综合征出血热患者流产胎儿肝脏分离病毒84FLi株的全M片段基因序列，了解其分子基础. 方法 采用反转录-聚合酶链反应(RT-PCR)，分节段扩增M基因片段的cDNA，直接用PCR产物或克隆入pMD18-T载体后进行测序. 结果 84FLi株的全M基因序列组成为:M片段基因共由3616个核苷酸组成，四种核苷酸的比例分别为A30.92%，C18.03%，G21.18%，T29.87%.GC含量为39.21%，AT含量为60.79%.最大开放读码框为41～3448，共编码1135个氨基酸.核苷酸序列同源性分析表明84FLi株与HTN型同源性高于与其他型汉坦病毒的同源性，其中与中国株的同源性较高，与HV114株的同源性为85.5%.与HTNV的国际标准毒株76-118的核苷酸同源性分别为84.0%，氨基酸的同源性为96.0%. 结论 84FLi株属于汉滩型病毒，并与分离自国内的汉滩病毒同源性较高.

    Sequencing and analyses of complete M segment se┐quence of84FLi，a Hantaan virus isolated from Xi'an

    LI Xin-Hong YANG Wei-Song HANG Chang-Shou BAI Xue-Fan MA Ben-Jiang HUANG Chang-Xing LI Guang-Yu

    1 Infectious Diseases Center of Chinese PLA，Tang-du Hospital，Fourth Military Medical University，Xi'an710038，China，

    2 Institute of Virology，Chi-nese Academy of Preventive Medicine，Beijing100052，China

    Keywords:hantaan virus;strain84FLi;sequence analysis;phylogenetic tree

    Abstract:AIM To study the complete M segment sequence of84FLi，a Hantaan virus isolated from a fetal liver in Xi'an and to explore its molecular characters.METHODS The cDNA of M segment was amplified fragment by fragment us-ing RT-PCR.The purified PCR products were sequenced di-rectly or cloned into pMD18-T vector and then sequenced.RESULTS The complete M segment of84FLi strain was composed of3616nucleotides with a single open reading frame ......