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编号:10925294
PAGE4单克隆抗体的制备及表达分布
http://www.100md.com 《第四军医大学学报》 2004年第23期
前列腺癌,,单克隆抗体;CT抗原;前列腺癌;PAGE4,0引言,1材料和方法,2结果,3讨论,【参考文献】
     bution of PAGE4 in normal and malignant tissues

    LAN Tian,CHEN LiHua,ZHUANG Ran,FANG Liang,TIAN Ying,XUE JiangNan,WANG Zhe,JIN BoQuan

    1Department of Immunology, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University,Xi’an 710033,China

    【Abstract】 AIM: To prepare antiPAGE4 monoclonal antibody and to analyze the expression of PAGE4 in normal tissues and some tumors. METHODS: BALB/c mice were immunized with recombinant PAGE4 protein and the hybridoma cell lines secreting monoclonal antibodies (mAbs) against PAGE4 were established using routine hybridoma technique. Western Blotting, ELISA and immunohistochemical staining were performed to identify the specificity and sensitivity of the PAGE4 mAbs as well as the expression of PAGE4 in normal tissues and some tumors. RESULTS: Four hybridoma cell lines secreting mAbs to PAGE4 with high specificity and sensitivity were obtained. PAGE4 was strongly expressed in spermatogonia and some of primary spermatocytes of testis, normal prostate and uterus in human reproductive tissues, and this molecule was also localized in normal pancreas. The expression of PAGE4 molecule, a novel member of Cancer/Testis antigen family, was observed in such tumor tissues as those of prostate adenocarcinoma, uterus adenocarcinoma and clear cell renal adenocarcinoma. CONCLUSION: The mAbs against PAGE4 were prepared which will facilitate further investigation of the distribution and function of PAGE4 molecule. ......

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