PAGE4单克隆抗体的制备及表达分布
前列腺癌,,单克隆抗体;CT抗原;前列腺癌;PAGE4,0引言,1材料和方法,2结果,3讨论,【参考文献】
bution of PAGE4 in normal and malignant tissuesLAN Tian,CHEN LiHua,ZHUANG Ran,FANG Liang,TIAN Ying,XUE JiangNan,WANG Zhe,JIN BoQuan
1Department of Immunology, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University,Xi’an 710033,China
【Abstract】 AIM: To prepare antiPAGE4 monoclonal antibody and to analyze the expression of PAGE4 in normal tissues and some tumors. METHODS: BALB/c mice were immunized with recombinant PAGE4 protein and the hybridoma cell lines secreting monoclonal antibodies (mAbs) against PAGE4 were established using routine hybridoma technique. Western Blotting, ELISA and immunohistochemical staining were performed to identify the specificity and sensitivity of the PAGE4 mAbs as well as the expression of PAGE4 in normal tissues and some tumors. RESULTS: Four hybridoma cell lines secreting mAbs to PAGE4 with high specificity and sensitivity were obtained. PAGE4 was strongly expressed in spermatogonia and some of primary spermatocytes of testis, normal prostate and uterus in human reproductive tissues, and this molecule was also localized in normal pancreas. The expression of PAGE4 molecule, a novel member of Cancer/Testis antigen family, was observed in such tumor tissues as those of prostate adenocarcinoma, uterus adenocarcinoma and clear cell renal adenocarcinoma. CONCLUSION: The mAbs against PAGE4 were prepared which will facilitate further investigation of the distribution and function of PAGE4 molecule. ......
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