Production of blastocysts by transfer of nuclei from nonquiescent adult rabbits bone mesenchymal stem cells and adipose stromal stem cells

    YAN Quan-Jian LI Liu-Jin YANG Gui-Zhong ZHANG Yu-Mei WANG Yue-Feng WANG He SHAO Guo-Xing

    Keywords:nuclear transfer;adult rabbits;bone mesenchy-mal stem cells;adipose stromal stem cells;oocytes donation;blastocysts

    Abstract:AIM To study the isolation and in vitro culture of rabbit's bone mesenchymal stem cells(BMSCs)and adipose stromal stem cells(ASSCs)and the use of both BMSCs and ASSCs cells in nuclear transfer(NT)to produce totipotent blastocysts.METHODS Both cell lines were derived from New Zealand rabbits and used as donor cells.The cell lines were cultured as a low cell density suspension in the Dulbecco's modified Eagle's medium(DMEM)and150mL·L-1 fetal calf serum(FCS)for7d before use in nuclear transfer.Direct intracystplasm microinjection techniques were used to reconstruct one-cell embryos.Sire differences were noted in the ability of both BMSCs and ASSCs to form blastocysts.RESULTS A total of986nuclear transfer clones were made by fusing both BMSCs and ASSCs cells nuclei into enucleated oocytes.After culture of the clones for7d in vitro ......