关键词: GABA;甘氨酸;延髓;免疫电镜;大鼠

    摘 要:目的 观察大鼠延髓背角(MDH，三叉神经脊束核尾侧亚核)浅层(Ⅰ，Ⅱ层)内降钙素基因相关肽(CGRP)阳性终末与γ-氨基丁酸(GABA)能和甘氨酸(Gly)能神经元的突触联系. 方法 包埋前GABA或Gly免疫组织化学染色方法结合包埋前CGRP免疫金标记电镜技术. 结果 GABA或Gly阳性产物广泛分布于神经元胞体、轴突和树突内，CGRP阳性标记主要见于无髓和薄髓轴突内.约53%和51%的CGRP阳性轴突终末分别与GABA和Gly阳性神经元的胞体或树突形成非对称性突触，其中的多数为轴-树突触，轴-体突触较少. 结论 含CGRP的初级传入纤维直接与MDH浅层内的GABA能及Gly能抑制性中间神经元形成突触联系.

    GABA┐and glycine┐immunoreactive neurons postsy┐naptic to calcitonin gene┐related peptide┐immunore┐active terminals in the superficial layers of the rat medullary dorsal horn

    FENG Yu-Peng，YANG Kun，LI Yun-Qing

    Department of Anatomy and K.K.Leung Brain Re-search Centre，The Fourth Military Medical Uni-versity，Xi'an710033，China

    Keywords:GABA;glycine;medulla oblongata;microscope，immunoelectron;rats

    Abstract:AIM To investigate the synaptic connections be-tween calcitonin gene-related peptide-immunoreactive(CGRP-ir)terminals andγ-aminobutyric acid(GABA)-and glycine(Gly)-ir neurons in the superficial layers(laminae I and II)of rat medullary dorsal horn(MDH).METHODS Pre-embedding immunohistochemical detection of GABA or Gly ...... 濠碘€冲€归悘澶愬箖閵娿儲韬ù锝堟硶閺併倝骞嶇€ｎ偅绨氱紒娑橆槹缁侊妇鎲撮崼鐔割槯闁哄啰濮电涵鍫曞蓟閵壯勭畽闁瑰瓨鐗旂粭鍛姜閽樺寮块柡鍌氭祫缁辨繈宕ｉ婵嗗幋闁哄嫷鍨甸～锕傚箹濠婂懎鍋嶇€殿喗娲橀幖鍛婂緞鏉堚晜鍩傞柍銉︾矎濞村棝鎯嶆担娴嬪亾濠垫挾绀夐悹鍥棑閸嬶綁宕欑拠鑼綄妤犵偞娲樺〒鑸电▔鐎ｎ偅鐓欓柣銊ュ閳ь剚绮庨弫鎼佹嚇閹寸姴顣奸柍銉︾箖閸ㄣ劑鍨惧⿰鍐ㄦ枾缂傚啯鍨块妴澶愬灳濠靛⿴鍟忛梻鍌ゅ枔閳ь剨鎷�


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