关键词: bax基因;癌，肝细胞;基因转染;基因表达

    摘 要:目的 建立稳定高表达Bax蛋白的肝癌细胞. 方法 用脂质体介导的基因转染法分别将含人bax cDNA的pBK-bax和pBK-CMV空载体质粒导入人肝癌细胞系HCC-9204细胞中.经G-418筛选获得细胞克隆.ABC法和图像定量分析检测Bax蛋白在细胞中的表达情况.HE染色观察细胞形态学变化.TUNEL法检测细胞凋亡发生情况. 结果 转染后用G-418筛选2wk，获得了细胞克隆.转染pBK-bax细胞的Bax蛋白表达强度明显强于转染pBK-CMV或未转染的HCC-9204细胞(P<0.05)，而转染pBK-CMV的细胞与未转染的HCC-9204细胞的Bax蛋白表达强度未见明显差异.pBK-bax转染的细胞有的可见细胞固缩、变圆、浓染和“出泡”等凋亡形态学变化，并且其TUNEL指数明显高于转染pBK-CMV或未转染的HCC-9204细胞(P<0.05). 结论 获得了稳定高表达Bax蛋白的肝癌细胞.bax基因转染的HCC-9204细胞凋亡率明显增高.

    Establishment of the hepatoma cells which express Bax protein stably and highly and observation of its apoptotic phenomena

    YANG Lian-Jun，WANG Wen-Liang

    Department of Pathology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:bax;carcinoma，hepatocellular;gene transfec-tion;gene expression

    Abstract:AIM To elucidate the adjusting function and op-erating mechanism of proapoptotic gene bax in the apoptotic process of hepatocellular carcinoma(HCC)，and to establish the HCC cells which can express Bax protein stably and high┐ly.METHODS The plasmid of bacteriophague expression vector pBK-bax ......