Effects of flavonoids extracted from core of Litchi Chinensis Sonn on HBsAg and HBeAg expression and HBVª²DNA content in HepG2.2.15 cells

    XU Qing, SONG Yunª²Juan, CHEN Quanª²Bin, YI Xiangª²Hui

    1Department of Pharmacology, Guilin Medical College, 2School of Chemistry & Chemical Engineering, Guangxi Normal University, Guilin 541004, China

    ¡¾Abstract¡¿ AIM: To study the antiª²HBV action of flavonoids ( IL ) extracted from the core of Litchi Chinensis Sonn.METHODS: Optical density mensuration, cell culture and FQ ª² PCR technique were used to study the inhibitory effects of IL extracted from the core of Litchi Chinensis Sonn on the expressions of HBsAg and HBeAg and the content of HBVª²DNA in HepG2.2.15 cells.RESULTS: In 96ª²hole plate test, IL (800£¬ 400£¬ 200£¬ 100 mg/L)had obvious inhibitory effects on the expressions of HBsAg and HBeAg in HepG2.2.15 cells on the third and sixth experiment days, compared with that of the control group (P<0.01). In 24ª²hole plate test, IL (400£¬ 200£¬ 100£¬ 50 mg/L) had obvious inhibitory effects on the expression of HBsAg on the third, sixth and ninth experiment days and on the expression of HBeAg on sixth and ninth experiment days compared with those of the control group (P<0.01). FQª²PCR showed that IL (400 mg/L) turned HBVª²DNA in HepG2.2.15 cells medium negative. CONCLUSION: IL has strong antiª²HBV action in vitro. ......