大肠菌群纸片法在食具消毒效果检测中应用的探讨

    孔 梅 天津市卫生防病中心 (300011)

    李 铮 红桥区卫生局公共卫生监督所(300132)

    摘要 大肠菌群检测一般采用多管发酵标准方法测定1

    ,自 1985 年 Forg 建立了纸片

    法2

    ,因其具有快速、简便等优点已在食具消毒效果中广泛应用 ,但有时纸片结果会模糊不清 ,不易判断 ,为了提高其检测结果的准确性以便在实际工作中更好的利用方法 ,本实验对大肠菌

    群纸片法与常规发酵法在食具消毒效果中进行比较试验。结果表明 ,对出现可疑结果的纸片

    放入乳糖胆盐培养液中再按常规发酵法的程序进行大肠菌群检测 ,可提高纸片法的灵敏度 ,增

    加与常规发酵法的阳性符合率。

    关键词 大肠菌群 快速检测纸片法 发酵法 阳性符合率

    Abstract Objective Coliform detection is usually tested by multiple tube fermentation method.

    Since 1985 ,slip metod which was invented by Frog has been widely applied in tableware sterilization ef2

    fects ,because it is fast and simple. However , sometimes the slip result blurs and becomes difficult to

    judge. In order to improve the accuracy of the test result ,so as to apply this metod better in practice ,this

    exper iment compares tableware sterilization effects between colifrom slip method and conventional fermen 2

    tation method. The results showed that when the slip method and conventional fermentation method. The

    results showed that when the slip which appers doubtable is placed in lactose bile cultivation liquid ,then

    tested the colifrom with the conventional fermentation method ,the sensitivity of slip method can be im 2

    proved and the match rate to the convention fermentation method is increased.

    Keywords Coliform Rapid slip method Fermentation method Positive match rate

    在卫生检测中最常应用于粪便污染指示菌的是

    大肠菌群 ,它是指一群能在 36 ℃,24h 内发酵乳糖产

    酸产气 ,需氧兼性厌氧的革兰阴性无芽胞杆菌 ......