摘要：目的：探讨血清总补体溶血活性(CH50)在临床中的应用价值。并观察室温下放置不同时间血清CH50的变化。方法：采用脂质体-免疫分析法检测50例健康成年人、14例慢性肝病患者和26例狼疮肾病患者(其中11例为活动期)血清的CH50及C3和C4含量。结果：结果表明健康成年人LIA法血清总补体溶血活性(CH50)范围为25～57U/ml。血清在室温下放置4h后, CH50明显降低。14例慢性肝病患者CH50及C3和C4较健康者降低，其中C3、 C4与CH50的相关系数为0.857和0.807。26例狼疮肾病患者活动期与非活动期C3、 C4含量无明显差异，而活动期较非活动期CH50明显降低，有极显著差异。结论：总补体溶血活性(CH50)检测，待测血清必需新鲜，室温放置不能超过4h。脂质体-免疫分析法(LIA)检测总补体溶血活性，主要反映补体经经典途径活化的活性。补体含量及活性联合检测对临床诊断意义更大。

    关键词： 血清总补体溶血活性； 脂质体-免疫分析法； 补体

    The Determination of Serum CH50 with Liposome Immunoassay and its Clinical Application

    WANG Zhi-ping, LI Jing-yuan, LIU Li-xin, et al

    (Union Hospital，Tongji Medical College, Huazhong University of Science and Technology,Hubei Wuhan 430022，China)

    Abstract： Objective： To investigate the clinical applicable value of serum total complement hemolysis activity(CH50) and to investigate the change of CH50 in different time in room temperature after serum being collected.Methods： CH50,C3 and C4 were quantitated in sera from 50 healthy volunteers,14 patients with chronic liver disease and 26 patients with lupous nephropathy with liposome immunoassay.Results： In sera from healthy volunteers, CH50 ranged from 25 to 50U/ml and decreased significantly after being laid for 4 hours. In sera from patients with chronic liver disease, CH50,C3 and C4 decreased significantly comparing to healthy volunteers and the correlation coefficient was 0.857 and 0.807 between CH50 and C3 and between CH50 and C4 respectively. There were no significant difference between the levels of C3 and C4 in sera from patients with activity lupous nephropathy and non-activity lupous nephropathy, but there were significant difference between the CH50 in sera from patients with activity lupous nephropathy and non-activity lupous nephropathy.Conclusion： To quantitate the CH50, It is necessary to use fresh serum and the laid time is less than 4 hours in room temperature. CH50 tested by with liposome immunoassay mainly reflects complement activity of classical pathway. The combination assay of concentration and activity of complement have more value for clinical diagnosis. ......