Screening inhibitor of Staphylococcal enterotoxin B from random circular 7ª²mer peptide library displayed on phage

    LI Hanª²Ping, ZHOU Hong, ZHENG Yuª²Ling, DENG Xiaoª²Hong, MA Ru, JIANG Yongª²Qiang

    Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China

    ¡¾Abstract¡¿ AIM: To obtain the specific peptide which can bind with SEB and inhibit SEB¬ðs enterotoxin activity by panning from the random circular 7ª²mer polypeptide libraries. METHODS: The affinity of peptide and the competitive activity of the synthetic peptide with that of antiª²SEB monoclonal antibody (McAb) were detected respectively by Phageª²ELISA and competition ELISA. Animal experiment was performed to assess the suppressive effect of the screened peptide on the toxicity of SEB and its enterotoxin. RESULTS: The circular 7ª²mer peptide obtained by panning suppressed the multiplication of spleen lymphocytes in mice. At the ratio of 160:1 (SEB: synthetic 7ª²mers peptide), the peptide protected the little cats from attack of enterotoxin and the survival rate of mice treated with the screened peptide was significantly higher compared with that of the control group. CONCLUSION: The obtained peptide specifically combines with SEB and suppresses its enterotoxin activity, which paves the way for the development of SEB inhibitors. ......