Detection of sjTRECs levels in murine T lymphocytes in process of maturation by realª²time quantitative PCR

    ZHANG Huaª²Hua, ZENG Yaoª²Ying, ZHAO Jingª²Xian, JIANG Xun, HE Xianª²Hui, LING Yi

    Insititute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632, China

    ¡¾Abstract¡¿ AIM: To analyze the signal joint T cell receptor rearrangement excision circles(sjTRECs)levels of murine thymocytes and spleen lymphocytes in the process of maturation and to evaluate the amount of na¢†ve T cell and thymic function. METHODS: sjTRECs levels were determined by the real time quantitative PCR (SYBR Green). RESULTS: The amount of na¢†ve T cells in thymus increased from the age of 1 wk to 6 wk, but at the age of 9 and 12 wk, it began to decrease. The amount of na¢†ve T cells increased continually in the spleen from the age of 1 wk to 12 wk. CONCLUSION: In the process of murine maturation, the T cell synthesis function of thymus begins to weaken at 9 wk. The thymic output function is continually enhanced at the maturation process. Realª²time PCR is successfully developed and utilized in analyzing the sjTRECs levels and thymic function. ......