基异黄酮类_____基异黄酮类；磁共振谱学；荧光光度测定法

7,4′二羟基异黄酮3′磺酸钠的定性和定量分析

http://www.100md.com 《第四军医大学学报》 2005年第13期

基异黄酮类,,基异黄酮类；磁共振谱学；荧光光度测定法,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Qualitative and quantitative analysis of sodium 7,4′bihydroxylisoflavone3′sulfonate

    ZHANG XiaoQing, QIN Bei

    Department of Pharmacy, Shaanxi Medical College, Xian 710068, China

    【Abstract】 AIM: To establish qualitative and quantitative analysis methods of 7, 4′bihydroxylisoflavone3′sulfonate. METHODS: Nuclear magnetic resonance (NMR) and fluorescence spectrum were used to analyze sodium 7, 4′dihydroxylisoflavone3′sulfonate. RESULTS: The 1H NMR and 13C NMR spectra of the title compound were reported for the first time. The new method of determination of sodium 7, 4′bihydroxylisoflavone3′sulfonate was established. Sodium 7, 4′bihydroxylisoflavone3′sulfonate had a strong fluorescence on the solution (pH = 9.00±0.02) with the max wavelength of λ = 310 nm and λ = 480 nm. The linear range and the detection limit of the method were 0-3.5 mg/L (r=0.9995) and 30 μg/L, respectively. The 1H NMR, 13C NMR and fluorescence spectrum offered a foundation for the qualitative and quantitative analysis of sodium 7, 4′bihydroxylisoflavone3′sulfonate. CONCLUSION: The 1H NMR and 13C NMR method, precise and economical using D2O as solvent, can be used to analyze sodium 7, 4′bihydroxylisoflavone3′sulfonate qualitatively and the method is economic. The fluorescence method, specific, simple, precise and sensitive, can be used to analyze the title compound quantitatively. ......

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