基因_____基因，bcl2(期刊论文)

反义bcl2寡核苷酸对半乳糖诱导的晶状体上皮细胞中Bcl2蛋白及细胞周期的影响

http://www.100md.com 《第四军医大学学报》 2005年第16期

基因,,基因，bcl2,；寡核苷酸类，反义；半乳糖；晶体,细胞学,0引言,1材料和方法,2结果,3讨论,【参

     Effect of antisense oligodeoxynucleotide bcl2 on expression of Bcl2 protein and cell cycle of lens epithelial cells induced by galactose

    GE ZhengLong, FAN Fang, LI ChangFu, LI HaiXiang, ZENG XiaoPing

    1Department of Biochemisty, Zunyi Medical College, 2Department of Ophthalmology, Affiliated Hospital, Zunyi Medical College 563003, China

    【Abstract】 AIM: To investigate the effect of antisense oligodeoxynucleotide bcl2 (bcl2 ASODN) on the expression of Bcl2 protein and the cell cycle of rabbit lens epithelial cells(LECs)induced by galactose. METHODS: From translation initiation region of bcl2 gene, oligonucleotides were synthesized and modified with phosphorothioate. Rabbit lens were treated with galactose and bcl2 ASODN. The influence of bcl2 ASODN on the level of Bcl2 protein expression and the cell cycle of LECs induced by galactose was detected by flow cytometry. RESULTS: 5.0-15.0μmol/L of bcl2 ASODN reduced the level of Bcl2 protein expression on LECs induced by galactose, with the fluorescence intensity decreasing from 158.70±2.14 to 113.94±2.88, and decreased the number of S phase cells of Lens from 20.60% to. 0.67%. CONCLUSION: bcl2 ASODN can inhibit the level of Bcl2 protein expression on LECs induced by galactose, interfere the entry of LECs into the S phase and inhibit the proliferation of LECs. ......

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