Induction effect of morselized bone on marrow stromal cells and treatment of rat cranial defect

    MA Song, YAN Jingª²Long, LIN Xin, PAN Haiª²Tao, WANG Xinª²Tao, YANG Xianª²Sheng

    1Department of Orthopaedics, Tiantan Hospital of Capital Medical University, Beijing 100050, China, 2Department of Orthopaedics, First Hospital of Haerbin Medical University, Haerbin 150001, China

    ¡¾Abstract¡¿ AIM: To investigate the induction effect of morselized bone on marrow stromal cells and to study the therapeutic effects of compound autogenetic minimal morselized bone and marrow stromal cells on rat cranial defect. METHODS: Rat marrow stromal cells (MSCs) were cultured and induced with morselized bone and mass bone. ALP, OCN, collagen ¦© expressions were semiquantified by RTª²PCR and immunohistochemical staining. The rat cranial defect model was prepared. The defect was repaired with compound autogenetic minimal morselized bone graft and marrow stromal cells. X ray examination was performed to observe the union. The survival of grafted cells was examined with BRDU immunohistochemical staining. RESULTS: After cultured with morselized bone, MSCs changed to osteoblast and the expressions of ALP, OCN and collagen ¦© in MSCs were significantly earlier and higher than those in mass bone group. The healing of cranial defect was more satisfactory in compound morselized bone and morrow stromal cells grafting group than that in morselized bone group and bromodeoxyuridine (BRDU) positive osteocytes were seen. CONCLUSION: After compound autogenetic minimal morselized bone and marrow stromal cells grafting, rat cranial defect repairs rapidly and the grafted cells survive. ......