Effects of constant magnetic field on secretion and expression of ICAMª²1 and VCAMª²1 in endothelial cells and their adhesion rates with monocytes

    ZHANG Zheng, LIU Yingª²Feng, CHENG Heª²Xiang, WANG Haiª²Chang, GUO Wenª²Yi, JIA Guoª²Liang, ZHOU Lian

    1Department of Cardiology, Zhujiang Hospital, South Medical University, Guangzhou 510282, China, 2Department of Cardiovasology, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, 3Northwest Institute for Nonª²ferrous Metal Research, Xi¬ðan 710016, China

    ¡¾Abstract¡¿ AIM: To investigate the effects of constant magnetic field on the secretion and expression of intercellular adhesion moleculeª²1 (ICAMª²1) and vascular cell adhesion moleculeª²1 (VCAMª²1) in human umbilical vein endothelial cells (HUVECs) and on their adhesion rates with THPª²1 monocytes induced by angiotensin¢ò (Ang¢ò). METHODS: The third passage of cultured HUVECs were used. There were six groups: one control group, one Ang¢ò (1¡Á10-6 mol/L) group, and four Ang¢ò groups respectively plus 1, 5, 10 or 20 gausses of constant magnetic field. Samples were collected 24 h after incubation with or without magnetic field. The secretion and expression of ICAMª²1 and VCAMª²1 were detected by ELISA and immunocytochemistry respectively. The adhesion rate between HUVECs and THPª²1 was counted. RESULTS: The secretion and expression of ICAMª²1 and VCAMª²1 significantly increased in Ang¢ò (1¡Á10-6 mol/L) group (P<0.05 vs control) compared with those in control group, while the secretion and expression of ICAMª²1 and VCAMª²1 in 1, 5, 10 and 20 gausses groups significantly decreased compared with those in Ang¢ò(1¡Á10-6 mol/L) group (P<0.05). The adhesion rates between HUVECs and THPª²1 significantly increased 24 h after incubation of HUVECs with Ang¢ò(1¡Á10-6 mol/L) (P<0.05 vs control) while the adhesion rates between HUVECs and THPª²1 in 1, 5, 10 and 20 gausses groups significantly decreased comparing with those in the Ang¢ò group (1¡Á10-6 mol/L) (P<0.05). CONCLUSION: One gauss to 20 gausses of constant magnetic field can antagonize the effects of Ang¢ò on HUVECs, decrease the secretion and expression of ICAMª²1 and VCAMª²1 in HUVECs and decrease the adhesion rates between HUVECs and monocytes. ......