Construction of eukaryotic expression vector of human TFF2 gene

    ZHANG Shaoª²Rong, YANG Xiaoª²Qiang£¬XING Rui£¬SONG Yuª²Gang£¬ CHEN Xueª²Qing

    Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

    ¡¾Abstract¡¿ AIM: To construct a eukaryotic expression vector of human TFF2 gene. METHODS: We assembled a TFF2 gene in pfu mix reaction system and cloned it to pGMª²T easy vector. The positive colonies were first confirmed by restriction enzyme digestion and sequencing and then were inserted to eukaryotic expression vector pcDNA3.1 and verified by enzyme digestion and sequencing. RESULTS: After TA colon of synthetic TFF2, the positive colonies were finally verified by sequencing, which were totally in line with the designed coding sequence of TFF2 gene. CONCLUSION: Eukaryotic expression vector of human TFF2 gene has been successfully constructed, which provides a basis for further investigation of gastric ulcer. ......