Effects of recombinant adenovirus encoding antisense cª²myc on proliferation and cell cycle of HLª²60 cells

    CHEN Long, CHEN Jieª²Ping, LIN Chen

    1Department of Hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China, 2National Laboratory of Molecular Oncology, Cancer Institute, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021, China

    ¡¾Abstract¡¿ AIM: To investigate the effect of recombinant adenovirus encoding antisense cª²myc (Adª²Ascª²myc) on the proliferation and cell cycle of HLª²60 cells and to study the molecular mechanism of the effects in vitro. METHODS: The HLª²60 cells were transfected with Adª²Ascª²myc and protamine sulfate. The growth of HLª²60 cells was observed by MTT assay and the changes of cell cycle were analyzed by flow cytometer in HLª²60 cells. The transcription of cª²myc was assessed by RTª²PCR and immunocytochemical technique was used to test cª²myc and proliferating cell nuclear antigen. RESULTS: The growth of HLª²60 cells was markedly inhibited by Adª²Ascª²myc. Flow cytometry analysis showed that the Adª²Ascª²myc transfection arrested HLª²60 cells in G0/G1 phase, reduced S phase and increased apoptosis rate. Transcription of cª²myc was inhibited in the transfected HLª²60 cells. Immunocytochemical detection demonstrated that the Adª²Ascª²myc transfection suppressed the expression of cª²myc and PCNA in HLª²60 cells. CONCLUSION: Adª²Ascª²myc can block cell cycle progression and inhibit the proliferation of HLª²60 cells in vitro. The biological effects of Adª²Ascª²myc may be associated with the expression of cª²myc gene and PCNA. ......