Factors affecting slide platelet aggregation test time

    PAN Jiª²Chun1, BI Weiª²Ru1, OUYANG Xiª²Lin1, LIU Jingª²Han1, LI Xiª²Jin1, LI Hui1, MA

    Shuª²Xuan, Dean P. Bonderman, GAO Daª²Yong

    1Department of Blood Transfusion, Chinese PLA General Hospital, Chinese PLA Clinical Blood Transfusion center, Beijing 100853, China, 2Analytical Control Systems, Inc. USA, Fishers, IN 46083, 3University of Washington, Seattle, WA 98195ª²2600, USA)

    ¡¾Abstract¡¿ AIM: To explore the factors which affect the slide platelet aggregation test (SPAT) time. METHODS: The SPAT of the following platelet rich plasma (PRP) was tested: ¢Ù PRP and the gradient diluted samples from eight healthy blood donors. ¢Ú Ten healthy blood donors¬ð PRP treated respectively at 40, 35, 30, 25, 20, 15, 10 and 5 degrees centigrade for thirty minutes. ¢Û Ten healthy blood donors¬ð PRP treated respectively with 0, 1, 2, 3, 4 and 5 percent dimethyl sulphoxide for thirty minutes. ¢Ü PRP of eight volunteers before and after a threeª²day administration of ASA in a dose of 100 mg/day stored at room temperature for 1, 2, 3 and 4 hours respectively. ¢Ý PRP of eight healthy donors with 0, 0.5, 1.0, 2.0 and 3.0 U/L heparin or EDTA respectively. RESULTS: ¢Ù The SPAT time was prolonged when the platelet count decreased and they had a significant linear relationship (r=0.996, P=0.004). ¢Ú No significant difference in SPAT time was detected when the PRP was stored at the temperature ranging from 20 to 40 degrees Centigrade, but significant delay was detected when the storage temperature was below 20 degrees Centigrade. ¢Û A significant dose dependent delay effect on SPAT was detected when dimethyl sulfoxide (DMSO) was added into PRP. ¢Ü No significant difference of SPAT was detected when PRP was stored at room temperature for four hours (P=0.815). ¢Ý No significant difference of SPAT was detected when heparin was added as anticoagulant and 0.1 g/L EDTA inhibited the platelet aggregation completely. CONCLUSION: The platelet count, the platelet storing temperature and the DMSO content in platelets will to some extent delay the SPAT time but they will not inhibit the platelet aggregation, with the SPAT time still within 180 seconds. ......