A study of association between the polymorphism of endothelial nitric oxide synthase (eNOS) gene 27 bp variable number of tandem repeats (VNTR)and restenosis after coronary stenting

    LI Hui, LI Chengª²Xiang, JIA Guoª²Liang, LUAN Rongª²Hua, LU Anª²Lin, WANG Haiª²Chang, GUO Wenª²Yi

    Department of Cardialogy, Xijing Hospital Fourth Military University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the association of the polymorphism of endothelial nitric oxide synthase (eNOS) gene 27bp variable number of tandem repeats (VNTR) with restenosis after coronary stenting in Chinese population. METHODS: ¢ÙOne hundred and five cases who underwent coronary stenting and coronary angiography at least 3 months after the procedure were enrolled. The information of clinical risk factors and procedureª²related factors was collected; ¢ÚGenotypes of 105 patients were determined by polymerase chain reaction (PCR)and agarose gel electrophoresis and gene sequenciong. ¢ÛFasting serum nitric oxide metabolite (NOx) and Endothelin (ET) were measured by nitrate reductase and radioim munoassay respectively. RESULTS: ¢ÙIn total 105 enrolled patients, 65 cases developed inª²stent restenosis, and the others were free from restenosis.¢ÚTwo alleles, containing 4and 5, and three genotypes namely 4/4ª²homozygous, 4/5ª²heterozygous, 5/5ª²heterozygous, were indentified in both the patients with restenosis and the patients without restenosis. ¢ÛThe overall distributions of either allele or genotype frequencies differed significantly between the two groups, the genotypes of the 4 repeats allel, 4/5ª²heterozygous and 4/4ª²homozygus were more frequent in the patients with restenosis group than without restenosis group. (P<0.05).CONCLUSION: The 4 repeats allele in eNOS gene might have a higher risk of restenosis after coronary stenting, The 4 repeats allele in eNOS gene may be induced the procedure of restenosis by reducing NO release of endothelium and damaging the endothelial function. ......