Effects of indoleª²3ª²acetic and horseradish on apoptosis of BXPCª²3 cells

    LIU Liª²Ying, SONG Tuª²Sheng, HUANG Chen, YANG Ling, LUO Yu, NI Lei, SI L¨¹ª²Sheng

    1Department of Biology £¦ Genetics, 2Key Laboratory of Environment £¦ Disease Correlation Gene, 3Institute of Immunopathology, College of Life Science and Technology, Xi¬ðan Jiaotong University, Xi¬ð an 710061, China

    ¡¾Abstract¡¿ AIM: To explore how indoleª²3ª²acetic (IAA) and horseradish peroxidase (HRP) affect the apoptosis and free radicals expression of BXPCª²3 cells. METHODS: Trypan blue exclusion test was used to determine the mortality of BXPCª²3 cells treated by IAA/HRP and in situ cell apoptosis detection kit was used to detect the apoptosis of BXPCª²3 cells. Biochemical methods were employed to measure the production of SOD and MDA in BXPCª²3 cells and the levels of freeª²radical in BXPCª²3 cells were measured by Laser confocal microscope. RESULTS: The number of apoptosis cells between control group and IAA/HRP group had distinct difference in a doseª²dependent pattern and the production of the SOD and MDA in BXPCª²3 cells had distinct difference between control group and IAA/HRP group. The production of free radicals in BXPCª²3 cells had distinct difference after administration of IAA/HRP in a timeª²and doseª²dependant manner. CONCLUSION: The free radicals induced by IAA and HRP may be involved in apoptosis of BXPCª²3 cells. ......