Allosteric communication network of coupling residues in GPCR class A rhodopsin like family

    TAN Xiaoª²Dan, LU Zhiª²Yong, DENG Qinª²Kai, JIANG Yong

    1Department of Medical Physics, School of Biomedical Engineering, 3Department of Pathophysiology and Key Laboratory of Functional Proteomics of Guangdong Province, Southern Medical University, 2Guangdong Vocational College of Mechanical & Electrical Technology, Guangzhou 510515, China

    ¡¾Abstract¡¿ AIM: To predict the allosteric communication network formed by a set of amino acids coupled with Leuª²125 in G protein coupled receptor (GPCR) class A rhodopsin like family. METHODS: Based on the multiple sequence alignment (MSA) of class A GPCR, we used statistical coupling analysis to obtain the statistical energy of amino acid positions in the multiple sequence alignment. We then extracted the subalignment containing only isoleucine at Leuª²125 and calculated the statistical coupling energy between each position and Leuª²125. RESULTS: Based on the statistical coupling energy, we figured out the amino acids positions coupled with Leuª²125. CONCLUSION: These amino acid positions, verified by some studies to be important positions in class A GPCR, constitute an allosteric communication network in GPCR class A rhodopsin like family. ......