Resveratrol induced apoptosis and cell arrest in cancer cells

    ZHU Qing, ZHANG Wangª²Gang, WANG Liª²Feng, WANG Fang, YANG Anª²Gang

    1Department of Hematology, Second Hospital, Xi¬ðan Jiaotong University, Xi¬ðan 710004, China, 2Department of Oncology, People¬ðs Hospital of Shaanxi Privince, Xi¬ðan 710068, China, 3Department of Biochemistry & Molecular Biology, School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To study the mechanism of resveratrol induced HepG2 and K562 cells apoptosis and cell arrest. METHODS: Cell morphological method and Annexin V technology were used to detect HepG2 and K562 cells apoptosis, flow cytometry (FCM) was used to detect the cell cycle and MTT was used to detect the medicine sensitivity of HepG2 and K562 cells. RESULTS: Apoptosis was seen in human liver cancer HepG2 cell treated with resveratrol and nuclear chromatine condensation and fragmentation were observed in HepG2 cells. Typical ladder patterns of DNA fragmentation were also observed. The highest rate of HepG2 cell apoptosis was 33.7% by Annexin V. Cell cycle stopped at S phase and cell apoptosis rate was 9.97% by FCM. Time and dose dependent effects of resveratrol to HepG2 cell were detected by MTT. Resveratrol had no effect on K562 cells. CONCLUSION: Resveratrol induces HepG2 cell apoptosis and inhibits the development of human liver cancer with a time and dose dependent effect. The resveratrolª²induced cell apoptosis is specific to cancer cells. ......