肝星状细胞__(期刊论文)

转化生长因子β1 抗体对肝星状细胞内钙离子浓度的影响

http://www.100md.com 《第四军医大学学报》 2005年第17期

肝星状细胞,,转化生长因子β1抗体；肝星状细胞；钙离子；显微镜检查,共焦；正交设计,转化生长因子β1抗体对肝星状细胞内钙离子浓度的影响,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effect of transforming growth factorβ1 antibody on intracellular calcium ion in cultured hepatic stellate cells

    XIAO YongHong, LIU DianWu, LI Qing

    Department of Epidemiology , Hebei Medical University, Shijiazhuang 050017, China

    【Abstract】 AIM: To study the effect of transforming growth factorβ1 (TGFβ1 ) antibody on intracellular calcium ion ([Ca2+]i) in hepatic stellate cells (HSC) and to explore the molecular mechanism in prevention and treatment of hepatic fibrosis and portal hypertension. METHODS: The activated HSC line was plated on small glass coverslips in 24well culture dishes at a density of 5×109/L and stimulated by carbon tetrachloride (CCl4)and TGFβ1 antibody for 24 h respectively. After the cells were loaded with Fluo3/AM, [Ca2+]i was measured by laser scanning confocal microscopy (LSCM). Orthogonal design was used to study the changes of [Ca2+]i in HSCs treated with different concentrations of CCl4 and TGFβ1 antibody in different order and at different interval time. RESULTS: The [Ca2+]i was significantly different from different concentrations of TGFβ1 and CCl4 and different orders of treatment (P<0.05), while no significance was found between different interval times (P>0.05). CCl4 sharply increased [Ca2+]i when the dosage ranged from 5 to 15 mmol/L (P<0.05), while 5 to 20 mg/L TGFβ1 antibody decreased [Ca2+]i (P<0.05). CONCLUSION: TGFβ1 antibody can remit the [Ca2+]i overloading in HSC. ......

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