Synthesis and identification of CMV branched peptide

    YANG Sai, LIU Tao, YANG Hao, HAN Fengª²Chan, CHEN Wuª²Ling

    1College of Life Science, Northwest University, Xi¬ðan 710069, China, 2Institute of Genetic Diagnosis, Department of Pharmacy, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To investigate the synthesis of human cytomegalovirus antigenic branched peptide. METHODS: The linear peptide and branched multiple peptide with human cytomegalovirus¬ð antigenic epitope were synthesized with solid phase chemistry respectively and purified by HPLC. The two peptides¬ð molecular masses were obtained by mass spectrum and then identified with quality control serums. RESULTS: The molecular mass of the linear peptide was 793.8 and that of branched multiple antigenic peptide was 7127.77, both consistent with the theoretic molecular mass. The branched peptide differentiated quality control serums properly through ELISA. CONCLUSION: The synthesis of cytomegalovirus antigenic branched peptide greatly facilitates the previous way for the preparation of human cytomegalovirus antigen and has the advantage of low cost and no potential biohazard. ......