甲基化_____双歧杆菌DNA；树突细胞；小鼠

含有非甲基化CpG的双歧杆菌DNA对小鼠骨髓树突状细胞成熟的影响

http://www.100md.com 《第四军医大学学报》 2006年第2期

甲基化,,双歧杆菌DNA；树突细胞；小鼠,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Stimulatory effect of bifidobacteria DNA containing unmethylated CpG motifs on maturation of bone marrowderived dendritic cells

    AN Wei, ZHAO MiaoQing, LI YaLin, LI Song, KANG Li, SONG WenGang

    1Analytic Laboratory, Department of Immunology, School of Basic Medicine, Taishan Medical College, Taian 271000, China, 2Pathology and Physiology Institute, Medical College, Shandong University, Jinan 250012, China

    【Abstract】 AIM: To explore the effect of bifidobacteria DNA containing unmethylated CpG motifs (CpG DNA) on the maturation of bone marrowderived dendritic cells (BMDC). METHODS: The genomic DNA of bifidobacteria was extracted and purified, and the methylated degree of CpG motifs was tested. Cell phenotypes and antigens uptake of BMDC were analyzed by flow cytometry. The levels of IL6, IL12(p40) and TNFα were detected by ELISA and T cell proliferation was detected by 3HTdR incorportation test. RESULTS: Treated with bifidobacteria DNA for 24 h, BMDC expressed a high level of Ia, CD40, CD86 and CD11c molecules, significantly increased the secretion of IL6, IL12 (p40) and TNFα and significantly enhanced the pro ......

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