Preparation of nanoparticles containing bclª²xL gene and its high efficient transfection in rat cardiac myocytes in vitro

    LIAO Hongª²Ying, XIONG Liª²Hua, GU Liª²Jia, YIN Xiangª²Bao, XIAO Songª²Hua, MENG Rongª²Sen3, CHEN Bo

    1Department of Cardioª²thoracic Surgery, Third Affiliated Hospital, Guangzhou 510630, China, 2Department of Cardioª²thoracic Surgery, 3Medical Research Center, Second Affiliated Hospital, Sun Yatª²sen University, Guangzhou 510120, China

    ¡¾Abstract¡¿ AIM: To prepare nanoparticles containing bclª²xL gene and to investigate the feasibility of delivering the gene into rat cardiac myocytes in vitro. METHODS: Nanoparticles were prepared by the double emulsionª²solvent evaporation technique. Its morphology and size were observed under scanning electron microscope and DNA protection was observed by DNase¢ñdigestion. After bclª²xL gene was transferred into cardiac myocytes by LipofectAMINE 2000 and nanosphere respectively, bclª²xL expression was detected by Westernª²blot and RTª²PCR. RESULTS: Nanoparticles, with diameter ranging 200ª²260 nm, were well packaged, stable and capable of slowly releasing gene. bclª²xL expression in cardiac myocytes increased 1 week after the transfection compared with that in the controls (P<0.05). CONCLUSION: The nanoparticles containing bclª²xL gene have been successfully prepared and the exogenous bclª²xL can efficiently express in cardiac myocytes. Nanoparticle is an optimal gene vector for cardiac myocytes. ......