关键词: 肺/外科学;肺气肿;脱噬作用;细胞分裂

    摘 要:目的 观察肺减容术前、后肺内细胞增殖与凋亡的变化，并探讨慢性缺氧时肺内细胞增殖与凋亡的关系. 方法 建立肺气肿动物模型，单侧施行LVRS(lung volume re-duction surgery).LVRS术前及术后4wk处死动物，取完整肺组织应用免疫组化及原位缺口末端DNA碎片标记技术(TUNEL染色)对肺内增殖与凋亡细胞定量计数. 结果 用TUNEL标记DNA片段技术在石蜡切片上可清楚显示肺组织内散在的凋亡细胞，其核染色质浓集呈荧光染色阳性，或形成新月形，增生的细胞则呈Ki-67核阳性反应.凋亡细胞、Ki-67核阳性细胞手术前分别为(5.9±1.8)%，(1.7±0.7)%;手术后分别为(3.6±1.2)%，(2.7±0.9)%，术后凋亡降低有统计学意义. 结论 LVRS术前由于慢性缺氧致肺组织细胞凋亡增加，肺内细胞凋亡与增殖的平衡失调，LVRS术后随着低氧血症改善，肺组织细胞凋亡与增殖逐渐接近正常值.

    Experimental study of lung cell proliferation and apoptosis in pre┐and post┐operative lung volume re┐duction surgery in rabbits

    XU Hong，WANG Yun-Jie，LIU Kun，GAO Kun-Xiang

    Department of Thoracic Surgery，Tangdu Hospital，Fourth Military Medical University，Xi'an710038，China

    Keywords:lung/surgery;pulmonary emphysema;apoptosis;cell division

    Abstract:AIM To observe the variation of lung cell prolif-eration and apoptosis before and after LVRS and to investi-gate the relationship between the lung cell proliferation and apoptosis during chronic anoxia.METHODS Pulmonary emphysema animal models were established and operated a-long-side LVRS.The animals were sacrificed at4wk after LVRS and lungs were removed.The lung cell proliferation and apoptosis were determined quantitatively and qualitative-ly by immunohistochemical and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL)tech┐niques.RESULTS TUNEL examination showed scattered apoptosis cells with brown nucleus and concentrated or cres-cent shaped nucleus chromatin under microscope.Proliferat-ed cells displayed Ki-67nucleus positive reaction.The apop-tosis cells and Ki-67nucleus positive cells were(5.9±1.8)%and(1.7±0.8)%before LVRS ......