关键词: p15基因;肺肿瘤;原位杂交

    摘 要:目的 研究抑癌基因p15mRNA在肺癌组织中的表达，揭示p15基因的失活与肺癌的发生和其组织类型的相关性. 方法 对病理确诊的93(非小细胞肺癌65，小细胞肺癌28)例肺癌病理组织切片，采用p15cDNA探针以原位杂交组织化学方法检测p15mRNA在肺癌组织中的表达水平. 结果 p15mRNA阳性表达于肺癌组织细胞质或细胞核中，在细胞质中可见到较多的棕黄色颗粒，而细胞核内棕黄色颗粒相对少见.p15mRNA的阳性表达率:非小细胞肺癌为46%(30/65)，其中鳞癌46%(23/50)，腺癌47%(7/15).小细胞肺癌20%(5/25).非小细胞肺癌p15mRNA阳性表达率高于小细胞肺癌;高分化的鳞、腺癌阳性表达率高于同组中的低分化的鳞、腺癌(P<0.05). 结论 p15基因的失活与肺癌的发生密切相关，而且其失活程度与肺癌组织类型的恶性程度呈负相关.

    Signficance of p15mRNA expression in lung cancer tissue

    QI Hao-Wen，HAO Guang-Hui，LI Huan-Zhang Department of Respiratory Disease，Xijing Hospi-tal，Fourth Military Medical University，Xi'an710033，China

    Keywords:p15gene;lung neoplasms;in situ hybridization

    Abstract:AIM To show the association of inactivation of p15gene in lung cancers with pathogenesis and variation of lung cancer tissues by studying the expression of p15mRNA in lung cancers.METHODS There were93cases，of which65were NSCLC and28were SCLC，confirmed by pathologi-caly.The expression of p15mRNA in pathological sections of lung cancer was examined using p15cDNA and in situ hy-hyidizatino-histochemistry method.RESULTS In93lung cancers ......