ILG1:A new p4bacteriophage┐like integrase found in a New York laboratory Escherichia coli strain

    TIAN Wen-Zhi BAI Hui-Ling MA Yuan-Fang KEVIN Chua Warren Strober Charles C.Chu

    Keywords:integrase;recombination，genetic;bacterio-phages;p4

    Abstract:AIM Immunoglobulin(Ig)class switch recombi-nation(CSR)in germinal center B cells results in the switch in expression from one Ig isotype(IgM)to another isotype(IgG，IgA，or IgE).Mechanisms involved in this event are not understood.To understand this，we have begun to clone and characterize gene(s)involved in CSR by PCR-based cD-NA representational difference analysis(cDNA-RDA).METHODS B cells from common variable immuodeficiency(CVI)patients which can not undergo CSR due to B cells in-trinsic defects，but those from normal control can.These two types of B cell were cultured at the same condition where Ig class switching was induced.RNA from both CVI patient B cells(Driver)and normal control B cells(Tester)was iso-lated and reverse-transcripted into cDNA respectively.After amplicons were prepared，cDNA-RDA was applied by sub-tracting tester amplicon with excess amount of driver ampli-con.After five rounds of cDNA-RDA ......