关键词: 邻苯二甲酰亚胺;异鲁米诺衍生物;合成;化学发光;化学发光免疫分析

    摘 要:目的 建立一种合成化学发光标记试剂6-[N-(4-氨基丁基)-N-乙基]氨基-2，3-二氢-1，4-酞嗪二酮(ABEI)的简便、经济的新方法. 方法 室温下用KOH将邻苯二甲酰亚胺转化为邻苯二甲酰亚胺钾盐，并进一步进行甲基化、硝化和还原反应，得到N-甲基-4-氨基邻苯二甲酰亚胺.N-(4-溴丁基)邻苯二甲酰亚胺与N-甲基-4-氨基邻苯二甲酰亚胺缩合之后，通过乙基化和肼解反应得到目的产物ABEI. 结果 按起始原料计算，ABEI的总收率为4.4%.经熔点、核磁、质谱、红外、元素分析等方法鉴定，各中间体及目的产物均与其分子结构相符. 结论 起始原料价廉易得，反应步骤操作简便，有利于降低反应成本.

    Synthesis of6┐[N┐(4┐aminobutyl)┐N┐ethyl]amino┐2，3┐dihydro┐1，4┐phthalazinedione

    KUANG Yong-Qing，SUN Xiao-Li，HE Wei，WEI Lin-Lin

    Department of Chemistry，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:phthalimide;isoluminol derivative;synthesis;chemiluminescence; chemiluminescent im-munoassay

    Abstract:AIM To develop a simple and economical new approach to the synthesis of6-[N-(4-aminobutyl)-N-ethyl]-amino-2，3-dihydro-1，4-phthalazinedione (ABEI).METHODS Phthalimide was treated with KOH at room temperature to form potassium phthalamide，which was con-verted to N-methyl-4-aminophthalimide by methylation ......