表达HBeAg和HBsAg对HepG2细胞系细胞因子分泌及ACE活性的影响
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刘志英, 黄玉波, 魏红山, 宋淑静, 冯 鑫,
HBV;肝纤维化;血管紧张素转化酶;HepG2,表达HBeAg和HBsAg对HepG2细胞系细胞因子分泌及ACE活性的影响,刘志英,黄玉波,魏红山,宋淑静,冯鑫,刘亚楠,成军,EffectsofHB
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刘志英, 黄玉波, 魏红山, 宋淑静, 冯鑫, 刘亚楠, 成军,北京地坛医院传染病研究所 北京市 100011
北京市优秀人才专项经费资助项目, No. 20041D0301548
通讯作者: 魏红山, 100011, 北京市东城区安外大街地坛公园13号, 北京地坛医院传染病研究所. drliver@163.com
电话: 010-64211031-2358 传真: 010-64211031-2306
收稿日期: 2006-01-13 接受日期: 2006-01-24
Effects of HBeAg and HBsAg on cytokine excretion and angiotensin-converting enzyme activity in HepG2 cell line
Zhi-Ying Liu, Yu-Bo Huang, Hong-Shan Wei, Shu-Jing Song, Xin Feng, Ya-Nan Liu, Jun Cheng
Zhi-Ying Liu, Yu-Bo Huang, Hong-Shan Wei, Shu-Jing Song, Xin Feng, Ya-Nan Liu, Jun Cheng, Institute of Infectious Disease, Beijing Ditan Hospital, Beijing 100011, China
Supported by Program for Excellent Talents in Beijing, No. 20041D0301548
Correspondence to: Hong-Shan Wei, Institute of Infectious Disease, Beijing Ditan Hospital, 13 Ditan Park, Anwai Street, Beijing 100011, China. drliver@163.com
Received: 2006-01-13 Accepted: 2006-01-24
Abstract
AIM: To evaluate the effects of hepatitis B virus surface and e antigens (HBeAg and HBsAg) on the activity of angiotensin-converting enzyme (ACE) and cytokines excretion in hepatocellular carcinoma cell line HepG2.
METHODS: The eukaryotic expression plasmids, including pcDNA3.1(-)-HBsAg, pcDNA3.1(-)-HBeAg, and empty plasmid pcDNA3.1(-), were transfected into HepG2 cells, and the levels of cytokines, such as IL-6 and IL-8, and the activity of ACE were determined in the supernatants 48 h after transfection by flow cytometry and biochemical methods, respectively.
RESULTS: The expression of HBeAg and HBsAg were detected in the supernatants, but there was no significant difference of ACE activity between the cells tranfected with pcDNA3.1(-)-HBsAg, pcDNA3.1(-)-HBeAg, and empty plasmid pcDNA3.1(-). The levels of IL-6 and IL-8 in the supernatants had no significant differences between the cells transfected with pcDNA3 ......
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