轮状病毒NSP4基因在非复制型腺病毒载体中的表达及免疫活性
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王大燕, 王健伟, 王彦斌, 魏 强, 屈建国,
轮状病毒;NSP4;腺病毒载体;免疫,王大燕,王健伟,王彦斌,魏强,屈建国,洪涛,王大燕,Generationandimmunogeni
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王大燕, 王健伟, 王彦斌, 魏强, 屈建国, 洪涛, 中国疾病预防控制中心病毒病预防控制所 北京市 100052
王大燕, 博士, 中国疾病预防控制中心病毒病预防控制所助理研究员, 主要从事分子病毒学研究.
国家自然科学基金资助项目, No. 30000145, 30370066
国家863计划资助项目, No. 2003AA215071
通讯作者: 王健伟, 100052, 中国疾病预防控制中心病毒病预防控制所, 现在北京东单三条9号, 中国医学科学院病原生物学研究所. wangjw28@vip.sina.com
电话: 010-63511368
收稿日期: 2006-02-07 接受日期: 2006-02-13
Generation and immunogenicity of a recombinant replication-incompetent adenovirus expressing human rotavirus NSP4 protein
Da-Yan Wang, Jian-Wei Wang, Yan-Bin Wang, Qiang Wei, Jian-Guo Qu, Tao Hong
Da-Yan Wang, Jian-Wei Wang, Yan-Bin Wang, Qiang Wei, Jian-Guo Qu, Tao Hong, Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China
Supported by National Natural Science Foundation of China, No.30000145, 30370066 and the Foundation of National 863 Project, No. 2003AA215071
Correspondence to: Dr. Jian-Wei Wang, Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China. wangjw28@vip.sina.com
Received: 2006-02-07 Accepted: 2006-02-13
Abstract
AIM: To investigate the efficacy of passive protection induced by the recombinant adeno-virus bearing human rotavirus NSP4 gene and to explore the clues for novel rotavirus vaccine development.
METHODS: Human rotavirus NSP4 protein was expressed in a recombinant adenovirus. AdEasy system was recruited to facilitate the preparation and the NSP4 gene was inserted into the early 1 region of the vector by homologous recombination in E.coli. The expression of NSP4 was confirmed by Western blotting. The resultant recombinant adenovirus rvAdEasyNSP4 was induced to mice by intranasal (i.n.) immunization. Specific IgG and IgA antibodies were detected in the murine serum. Murine pups born to the rvAdEasyNSP4 immunized dams were challenged with simian rotavirus SA11 strain orally 4 d after birth, and the induced diarrhea was graded ......
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