[摘要] 目的 观察破格救心汤对心肌梗死后心力衰竭大鼠心肌细胞凋亡的影响。方法将Wistar大鼠手术建立心力衰竭模型后随机分成破格救心汤组(12只)、卡托普利组(12只)、模型组(12只)，另选未经手术的Wistar大鼠12只作为对照组。破格救心汤组每日2次灌胃破格救心汤10mL/kg;卡托普利组每日2次灌胃卡托普利液10mL/kg，模型组和对照组每日2次灌胃饮用水10mL/kg。每周称体质量1次，据此调整用药量。在实验第3周、第6周分别将大鼠心脏制成石蜡切片。采用原位末端标记(TUNEL)法检测心肌细胞凋亡，免疫组化法检测凋亡调控基因bax及bcl-2蛋白表达。结果 破格救心汤组、卡托普利组动物心肌梗死边缘区凋亡阳性细胞较模型组明显减少，给药6周后，凋亡率更低。破格救心汤组中，bax蛋白表达平均光强度显著升高，bcl-2蛋白表达平均光强度明显降低。结论 破格救心汤对心肌梗死后心力衰竭心肌细胞凋亡有改善作用，这可能与其对细胞凋亡调控基因的改变有关。

    [关键词] 破格救心汤;心力衰竭;细胞;凋亡

    Influence of Poge saving heart decoction on myocardial cell's apoptosis of heart failure rat

    LIANG Yi-qiang，CHEN Yuan-ping

    (1.Guangxi Traditional Chinese Medical College，Nanning530001，Guangxi，China;2.The First Affiliated Hospital of Guangxi Traditional Chinese Medical College，Nanning530001，Guangxi，China)

    Abstract:Objective It is to observe the influence of Poge saving heart decoction(PSHD)on myocardial cell's apoptosis of heart failure rat after myocardial infarction.Methods The heart failure models were made with36Wistar rats and random-ly divided into PSHD group(12rats) ......