[摘要]目的 评估氨磷汀(amifostine，AMF)在保护造血干.祖细胞免受化疗药物依托泊甙(etoposide，VP.16)损伤方面的作用。 方法 提取新鲜和冻存的外周血造血干细胞(PBSC)和HL.60细胞，分为AMF+VP.16组、VP.16组和空白对照组，用台盼蓝拒染法检测细胞活性，CFU.GM集落培养计数，流式细胞仪测定细胞的凋亡率。 结果 新鲜和冻存的PBSC样本中，AMF+VP.16组的细胞存活率和集落形成能力较VP.16组显著提高(均P<0.05);HL.60细胞样本中，AMF+VP.16组与VP.16组凋亡率的差异无统计学意义(P>0.05)。 结论 AMF能保护造血干.祖细胞免受化疗药物VP.16的损伤，并且不影响VP.16对HL.60细胞的杀伤效果。

    [关键词] 氨磷汀;细胞保护剂;造血干.祖细胞

    The protective effects of amifostine on hematopoietic stem.progenitor cells against the chemotheraputic damage

    ZHOU Min，CHEN Bao-an，LI Cui-ping，GAO Chong，DING Jia-hua，SUN Yun-yu，GAO Feng，SHAO Ze-ye

    (Department of Hemotology，Zhongda Hospital，Southeast University;Central of Mycelodysplastic Syndrome，the Medical College，Southeast University，Nanjing210009，China;Red Cross Blood Center，Nanjing210011，China)

    Abstract:Objective To study the protective effects of amifostine(AMF)on hematopoietic stem.progenitor cells against the damage of chemotheraputic drug—etoposide(VP.16).Methods Fresh and frozen peripheral blood stem cells(PBSC)and HL.60cells were collected and divided into the AMF+VP.16group ......