【摘要】 目的 研究草栽和段木栽培灵芝的多糖性质和含量; 考察以草代木栽培灵芝可行性的研究。方法 提取、分离、逆相透析方法研究二者子实体多糖。应用气相色谱、凝胶渗透色谱法测定多糖的组成、摩尔比和相对分子质量。IR、1H-NMR 和13C-NMR 等光谱确定多糖肽化合物结构。结果 草栽灵芝多糖肽(GLPG) 和段木多糖肽(GLPW ) 的重均相对分子质量(Mw) 分别为5.13×105 和5.85×105; 黏均相对分子质量(Mη)分别为6.21×105和6.96×105,二者具有相同的相对分子质量分布及光谱数据。从光谱分析中看出各单糖间的苷键构型相似, 均以β-苷键为主; 糖肽部分均含有相同的16种氨基酸, 糖的组份为鼠李糖、木糖、果糖、半乳糖、葡萄糖等, GLPW 还含有微量甘露糖。结论 草栽灵芝与段木灵芝多糖肽的主要理化特性相似,为草栽灵芝的综合开发、节约木材、保护生态环境与药用提供了科学依据。

    【关键词】 草栽灵芝多糖肽; 段木灵芝多糖肽; 提取; 纯化; 光谱鉴定

    Isolation and identification of active components of Ganoderma lucidum cultivated with grass and wood log (Extraction, purification and characterization of glycopeptide)

    LIN Shu-qian,WANG Sai-zhen,LIN Zhi-bin,et al.

    Fuzhou Green Valley Institute of Bio-pharmaceutical Technology, Fuzhou 350003,China

    【Abstract】 Objective To study the amount and properties of glycopeptide of Ganoder-ma lucidum ( Leyss.ex Fr. ) Kaist cultivated with grass ( GLPG ) and wood log ( GLPW ) ; to investigate the feasibility of substitution of grass for wood log to cultivate G. lucidum.Methods Glycopeptides were extracted and isolated from the f ruitbody of G. lucidum foll-owed by dialysis; themonosaccharid constituent and relative molecular weight of polysaccha-rides were examined by GC and gel filtration; structure of glycopeptide was determined by IR, 1H-NMR 和13C-NMR.Results Relative molecular weight s of GLPW and GLPG glycol-peptides were (M w ) 5.13×105 and 5.85×105, (Mη) 6.21×105 and 6.96×105, respect-tively; the glucosidic bonds of both glycopeptides wereβ-type; both glycopeptides consist 16 amino acids and Rha, Xyl, Fru, Gal and Glc ( there was trace amount of Man in GLPW).Conclusion There are less physical and chemical difference between GLPG and GLPW. The purification rate of GLPG is 2.8 times to GLPW. ......