摘要：目的 制备光敏剂血卟啉(HP)-单克隆抗体赫赛汀的光敏免疫偶联物，并观察偶联物对BT-474人乳腺癌细胞的杀伤及凋亡作用。 方法 以碳二亚胺为缩合剂，化学制备HP-赫赛汀光敏免疫偶联物。给予波长630 nm激光照射后，用MTT法、FCM检测比较免疫偶联物和游离HP对BT-474人乳腺癌细胞的杀伤及凋亡作用。 结果 含同剂量HP的偶联物较游离HP对BT-474细胞的杀伤及凋亡效果明显增强(P<0.05)。 结论 成功制备HP-赫赛汀光敏免疫偶联物，该偶联物对BT-474人乳腺癌细胞的杀伤作用强于游离HP。

    关键词：光免疫疗法；血卟啉；赫赛汀；光敏免疫偶联物；BT-474；乳腺肿瘤

    Preparation of hematoporphyrin-herceptin photoimmunoconjucate for photoimmunotherapy

    CHEN Lu, LUO Rong-cheng, LI Li-bo, YAN Xiao, DING Xue-mei

    Center of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

     Abstract: Objective To prepare the photoimmunoconjucate of hematoporphyrin (HP) and herceptin, and study its killing and apoptosis-inducing effect on tumor cells BT-474. Methods HP-herceptin photoimmunoconjugate was synthesized with EDCI as the condensator. After exposure of the cells to 630 nm laser, the killing effect of the conjugate and cell apoptosis were evaluated by MTT assay and flow cytometry. Results Compared with free HP at equivalent dose, the immune reactivity, killing effect and the apoptosis-inducing effect of HP-herceptin immunoconjugate on BT-474 cells was enhanced (P<0.05). Conclusion The killing effect of HP-Herceptin immunoconjugate is stronger than free HP on BT-474 cells. ......