【摘要】 目的 探讨外源性基质金属蛋白酶(matrix metalloproteinases，MMPs)抑制剂强力霉素在移植肺缺血-再灌注损伤中细胞凋亡的影响。 方法 建立大鼠自体左肺肺缺血再灌注模型，16只SD受体大鼠随机分为两组:缺血再灌注组和强力霉素组，用免疫组化方法观察移植肺Bcl-2、Bax和Caspase-3表达情况，并取肺组织平滑肌细胞进行培养，采用荧光技术测定肺组织细胞凋亡。结果 肺缺血再灌注后，与对照组相比，强力霉素组移植肺Bax和Caspase-3表达明显降低(P<0.01)，Bcl-2 的表达无明显变化(P>0.05)，但Bcl-2/Bax比例升高；荧光图片显示，强力霉素组的细胞凋亡明显较对照组减少。结论 强力霉素可能通过下调Bax、Caspase-3表达，提高Bcl-2/Bax比例而抑制肺缺血再灌注损伤的细胞凋亡，并通过降低基底膜的降解，减轻肺水肿，达到肺保护作用。

    【关键词】 肺； 缺血-再灌注损伤； 强力霉素

    Effect of doxycyline on pneumocyte apoptosis in ischemia-reperfusion injury of lung transplantation

    SHU Sheng-qiang，LIN Hui-qing，HUANG Jie.

    Renmin Hospital of Wuhan University，Wuhan 430060，China

    【Abstract】 Objective To investigate the effect of exogenous matrix metalloproteinase inhibitor on pneumocyte apoptosis in lung ischemia-reperfusion injury and the mechanism. Methods Models of ischemia-reperfusion lung injury in SD rats were randomly divided into two groups. The protein level expression of Bcl-2 、Bax and Caspase-3 was measured by immunohistochemistry methods after 2h transplantation. Apoptotic morphological changes were assayed with Hoechst 33258 staining and were observed under fluorescent microscope. Results The protein level expression of Bax and Caspase-3 was significantly decreased in doxycycline group than in control group (P<0.01) ......