海藻提取物抑瘤活性及抗氧化作用的观察
松节藻提取物,,],松节藻提取物；抑瘤活性；DNA氧化损伤；超氧化物歧化酶；丙二醛,1材料与方法,2结果,3讨论,[参考文献]
[摘要] 目的 探讨松节藻提取物对接种S180肉瘤细胞株小鼠的抑瘤作用以及抗氧化作用。方法 采用Horn法检测松节藻提取物的安全性(LD50)。给予荷瘤鼠喂饲25、50、100 mg/kg松节藻提取物，并设空白及阳性对照组，测定抑瘤率。以体内实验进行提取物的抗氧化活性评价，采用单细胞凝胶电泳法测定淋巴细胞的DNA损伤；以试剂盒测定血浆中超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果 松节藻提取物的LD50>3 160 mg/kg，属于低毒；松节藻提取物各个剂量组的平均瘤质量明显低于空白对照组，其中50 mg/kg剂量组的抑瘤率达到36.1%；松节藻提取物25 mg/kg剂量组对10 μmol/L的H2O2诱导的DNA氧化损伤有保护作用，且血浆SOD活性增强，MDA含量下降。结论 松节藻提取物为低毒化合物，使用安全；松节藻提取物可有效抑制S180肉瘤的生长；松节藻提取物具有良好的抗氧化作用。[关键词] 松节藻提取物；抑瘤活性；DNA氧化损伤；超氧化物歧化酶；丙二醛
ANTICANCER AND ANTIOXIDANT ACTIVITIES OF SEAWEED EXTRACTS
DONG CHUNJING，HE JUAN，LIANG HUI， et al
(Department of Nutrition, Qingdao University Medical College, Qingdao 266021, China)
[ABSTRACT]ObjectiveTo investigate the antitumor and antioxidant activities of various dosage of ethanol extract of R. confervoides (EERC) on mice. MethodsThe half lethal dose (LD50) of EERC was estimated by Horn assay. Sarcoma180 mice were used to evaluate the antitumor activities in vivo of EERC (Cytoxan as positive drug). The antioxidant activities of EERC were detected on mice. The oxidative DNA damage of peripheral lymphocytes induced by H2O2 was analyzed by SCGE. The concentration of superoxide dismutase (SOD), malonyl dialehyde (MDA) in blood plasma were detected with the test kit. ResultsThe EERC showed low toxicity with its LD50 more than 3 160 mg/kg. The tumor inhibition ratios of supplement groups were significantly higher than the blank group. There was a significant decreasing tendency of the oxidative DNA damage on peripheral lymphocytes in the 25 mg/kg supplement group induced by 10 μmol/L H2O2. The level of SOD in the 25 mg/kg supplement group was more than the control group, while its MDA was less than the control group. ConclusionThe EERC is safe to be taken orally and can obviously inhibit the sarcoma180. The 25 mg/kg EERC can protect the animal from oxidative damage. ......
您现在查看是摘要页，全文长 10308 字符。