银杏叶提取物对实验性脊髓损伤后细胞凋亡的影响
脊髓损伤,,],银杏叶提取物；脊髓损伤；细胞凋亡；大鼠,1材料和方法,2结果,3讨论,[参考文献]
[摘要] 目的 观察银杏叶提取物(GBE)对脊髓损伤后神经细胞凋亡的影响。方法 选择SD雄性大鼠(SPF级)30只，随机分成对照组和GBE组，均采用改良的Allen打击法制成中度急性脊髓损伤模型，术后GBE组给予GBE 0.75 mL/(kg·d)，对照组给予等量生理盐水。分别于术后1、3、5、7、14 d处死动物取材，采用流式细胞仪检测伤段脊髓细胞凋亡的情况。结果 对照组和GBE组均发现凋亡细胞，均在5 d达到高峰，但GBE组凋亡细胞比率显著低于对照组(t=4.97~9.56，P<0.05)。结论 GBE能够减少神经细胞的凋亡；抗凋亡可能是GBE缓解急性脊髓损伤的诸多机制之一。[关键词] 银杏叶提取物；脊髓损伤；细胞凋亡；大鼠
ANTIAPOPTOSIS EFFECT OF GINKGO BILOBA EXTRACT ON RATS WITH EXPERIMENTAL SPINAL CORD INJURY
ZHANG ZHONGLI, LI XIN
(Department of Orthopedics, Peoples Hospital of Weifang,Weifang 261041, China)
[ABSTRACT]ObjectiveTo investigate the antiapoptosis effect of Ginkgo biloba extract(GBE) after the spinal cord injury in SD rats.MethodsThirty SD male rats were randomly divided into two groups: control group and GBE group. The spinal cord injuries of the rats were induced by Allens way by a 5 g×5 cm impact on the posterior T9 spinal cord in moderate degree. The rats in the control group were treated with normal saline, while those in the other group were administrated GBE 0.75 mL/ (kg·d). The rats were sacrificed at 1, 3, 5, 7, 14 d after the injury. The cell apoptosis of the injured spinal cord was examined by flow cytometry. ResultsPositive expression of apoptotic cells appeared in both groups and reached their peak on third day after the injury. The cell apoptosis of the GBE group was significantly lower than that of the control group (t=4.97-9.56 ......
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