【摘 要】 目的 测定复方补骨脂酊中补骨脂素和异补骨脂素的含量。 方法 采用YWGC18色谱柱(4.6mm×200mm)，流动相为54%甲醇(含1%冰醋酸溶液)，流速为0.7ml/min，检测波长为295nm。 结果 补骨脂素和异补骨脂素保留时间分别为9.165min和10.082min，标准曲线的线性范围均为20～200mg/l(补骨脂素r=0.9999;异补骨脂素r=0.9993)。补骨脂素和异补骨脂素中的高、中、低、三种浓度溶液的日间和日内变异均小于10.0%，加样回收>90%。测定复方补骨脂酊中补骨脂素和异补骨脂素的含量分别为2.058、2.270、2.201mg/ml和1.665、1.617、1.609mg/ml。 结论 本法简便、快速重现性好，可用于复方补骨脂酊制剂质量控制的含量测定方法。

    【关键词】 复方补骨脂酊;补骨脂素;异补骨脂素;含量测定;高效液相色谱法

    Determination of psoralen and isopsoralen angelicin in compound Buguzhi tincture by HPLC method LIU Min，NIAN Shi-heng.(Bengbu Third People's Hospital，Anhui233000，China)

    【Abstract】 Objective HPLC method for determination of the contents of psoralen and isopsoralen angelicin in compound Buguzhi tincture was employed.Methods The HPLC analysis was made on a column of YWG C18(4.6mm×200mm)with a mobile phase of54%methanol(1%acetic acid glacid solution contained).The detection wavelength was at295nm and the flow rate was0.7ml/min.Results The retention time of psoralen and isopsoralen angelicin were9.165min and10.082min respec-tively.The linearity range of psoralen(r=0.9999)and isopsoralen angelicin(r=0.9993)were between20to200mg/l.The with-day and between-day RSD for psoralen and isopsoralen angelicin at different concentration was less than10.0%with average re-covery rate more than90%.The content of psoralen and isopsoralen angelicin in compound Bugnzhi tincture was2.058 ......