Cloning of collagen type¢ñgene and its expression in lens epithelium in normal and ageª²related cataractous people

    LIU Yan1, ZHOU Jian2 ,LIU Xinª²Ping3, YAO Liª²Bo3, SU Jingª²Kuan4, HUI Yanª²Nian2

    1Department of Ophthalmology , Tangdu Hospital, Xi¬ðan 710038,China,2Department of Ophthalmology, Xijing Hospital, 3Department of Biochemistry & Molecular Biology, School of Basic Medicine, Xi¬ðan 710033,China, 4Administration of Medical Education, Tangdu Hospital, Fourth Military Medical University , Xi¬ðan 710038,China

    ¡¾Abstract¡¿ AIM : To clone the gene of collagen type ¢ñ, to study its expression in lens epithelium of normal and ageª²related cataractous people, and to compare the difference of their expression. METHODS: The collagen type ¢ñ gene was cloned by the improved subtractive hybridization, and the fragment of collagen type ¢ñ cDNA was labeled by digoxin and was hybrided by in situ hybridization on the section of capsule of normal and cataractous lens. RESULTS: Collagen type I gene expressed both in the normal and the ageª²related cataractous lens epithelium. The positive cell rate was respectively (73¡À14ª±9)% and (75¡À16ª±3)%, the positive signals index was respectively(0ª±60¡À0ª±15) and (0ª±73¡À0ª±16) and the expression of the two groups had no significant statistics difference. CONCLUSION: Collagen type ¢ñ gene is expressed in the normal and the ageª²related cataractous lens epithelium. There is no significant differences in the expression of collagen type ¢ñ gene in the two groups, indicating that collagen type ¢ñ gene may play an important role in the etiology of cataract and the formation of posterior capsule opacification after cataract surgery. ......