膀胱癌_____膀胱癌；UROC28基因；克隆；原核表达

克隆人膀胱癌UROC28基因及其原核表达

http://www.100md.com 《第四军医大学学报》 2005年第3期

膀胱癌,,膀胱癌；UROC28基因；克隆；原核表达,0引言,1材料和方法,2结果,3讨论

     Cloning and prokaryotic expression of UROC28 gene of human bladder cancer

    YANG LiJun1, WU YuanMing2, WANG He1, YUAN JianLin1, LIU LiWen3, LIU ShuJuan4, SHAO GuoXing1, CHEN SuMin2

    1Department of Urology Surgery, Xijing Hospital, 2Department of Biochemistry and Molecular Biology, School of Basic Medicine, 3Department of Ultrasonic Diagnosis, Xijing Hospital, 4Department of Gynecology and Obstetrics, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To clone UROC28 gene of human bladder cancer and to express it in E. coli DH5α. METHODS: The fragment of UROC28 gene was amplified by RTPCR from human bladder cancer tissue and cloned to pUC19. The DNA fragment from pUC19UROC28 digested with HindⅢ and EcoRⅠ was ligated to XhoⅠ/EcoRⅠdigested prokaryotic expression vector pGEX4T1. The expression of fusion protein was induced with IPTG and the expressed product was identified by SDSPAGE. RESULTS: ① The sequencing and endonucleases digestion analysis showed that the fragment of UROC28 gene cDNA was inserted into the vectors pUC19 and pGEX4T1; ② SDSPAGE showed that UROC28 gene was expressed in E. coli DH5α. CONCLUSION: We have successfully constructed the recombinant plasmid of the UROC28 gene and successfully expressed the UROC28 gene, which will help further researches on the function of UROC28 gene and the preparation of antibodies to UROC28 protein. ......

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