High expression of transaldolase activity in human hepatoma

    ZHANG Rui, ZHANG Yiª²Ming, MENG Xiaoª²Xin, ZHOU Jinª²Yong, GU Dongª²Min, DE Wei

    Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, China

    ¡¾Abstract¡¿ AIM: To compare the activity of transaldolase (TAL) in hepatoma and normal liver tissues and to investigate the role of TAL in the formation of hepatoma. METHODS: Total protein was extracted from the liver tissue of 20 patients with hepatoma and 10 healthy controls, and the activity of TAL was evaluated. The TAL expressions at mRNA level were determined by RTª²PCR and those at protein level were determined by Western blot. RESULTS: The activity of TAL in hepatoma tissue [(674¡À44)nkat/g] was significantly higher than that in normal liver tissues [(287¡À44) nkat/g] and pericancerous tissues [(401¡À29) nkat/g] (P<0.05). The expression of TAL mRNA and protein was not significantly different between hepatoma tissues and normal liver tissues. CONCLUSION: The activity of TAL in hepatoma was significantly higher than that in normal liver tissue, which may be closely related to the neoplastic abnormalities. The change of activity may be associated with postª²translational modification. ......