Construction and expression of prokaryotic expression vector of human microtubule destabilizers Oncoprotein18

    YAO Qingª²Jun1, HU Daª²Hai1, CHEN Bi1, XU Mingª²Da1, DONG Maoª²Long1, JIA Chiª²Yu1, WANG Shuª²Sen2

    1Department of Burn Surgery, 2PLA Institute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To construct and express prokaryotic expression vector of human microtubule destabilizers Oncoprotein18 (Op18) and to prepare monoclonal antibody (mAb) against Op18. METHODS: Full length sequence of Op18 gene was amplified from human skin by RTª²PCR and was clones into the expression vector pRSET A. Op18 fusion protein was expressed in E.coli under IPTG induction and the expressed fusion protein was purified by glutathione agarose chromagraphy. RESULTS: The recombinant plasmid containing the target gene was constructed successfully. The fusion protein was expressed in E.coli in soluble form. CONCLUSION: The fusion protein is expressed in soluble form, which is a useful reagent for further preparation of monoclonal antibody and study of the function of Op18 in wound healing and scar excess. ......