cnExpression of RNA editase of mouse lymphocytes in a coª²culture system

    KONG Yaª²Lin,DOU Keª²Feng, ZHANG Hongª²Tao, FENG Quanª²Xin, ZHANG Fuª²Qin,ZHAO Qingª²Chuan

    Department of Hepatobiliary Surgery£¬Xijing Hospital£¬Fourth Military Medical University£¬Xi¬ðan 710033£¬China

    ¡¾Abstract¡¿ AIM: To study the expression of RNA editase of mouse lymphocytes under the effect of heterogeneous antigen in a coª²culture system of mouse spleen cells and human liver cancer cells and to observe the relation between RNA editase and the functional status of lymphocytes by the use of FK506. METHODS: Spleen cells of 1, 2, 4, 6, 8, 10, 12, 16, 20 and 24 h in the coª²culture system were observed and the expression of RNA editase was detected by semiª²quantitative PCR and was contrasted with the mouse spleen cells in a singleª²culture system. The mouse model with the immune system restrained by FK506 was made, its spleen cells were coª²cultured with stimulated cells and then the effect of FK506 on RNA expression of editase was detected by semiª²quantitative PCR. RESULTS: ¢Ù The lymphocyte survival rate within 24 h in each group reached 70%. ¢Ú The expression of RNA editase in the coª²culture group tended to show a pattern of declining, ascending and declining again, while that in the control group tended to decline constantly until 12 h and then remained at a comparatively low level. ¢Û FK506 had an obvious suppressive effect on the expression of RNA editase and this effect declined as the coª²culture time went on. CONCLUSION: The expression of RNA editase becomes active when stimulated by heterogeneous antigen and the expression level is related with the functional status of spleen cells. ......