Correlation between bclª²2/IgH gene rearrangement and GCB molecular subtype in diffuse large Bª²cell lymphoma

    JIANG Huiª²Yong, CHEN Yu, ZHANG Sanª²Quan, ZHU Meiª²Gang, ZHAO Tong

    Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

    ¡¾Abstract¡¿ AIM: To explore the correlation between bclª²2/IgH gene rearrangement and germinal center Bª²cellª²like (GCB) molecular subtype in diffuse large Bª²cell lymphoma (DLBCL). METHODS: Selfª²designed heminested PCR was used to detect bclª²2/IgH gene rearrangement in 60 cases of DLBCL. Positive PCR products were cloned and sequenced. The tissue microarray of 60 specimens of DLBCL was prepared and the expressions of CD20, CD10, Bclª²6 and MUM1 were investigated by immunohistochemical SP method. GCB and nonª²germinal center Bª²cellª²like (nonª²GCB) were subclassified. RESULTS: Six of the 60 DLBCL were bclª²2/IgH positive by conventional PCR method. Selfª²designed heminested PCR was used to amplify 6 positive cases again and bclª²2/IgH gene was verified rearrangement in 5 cases by cloning and sequencing and 1 had the gene segment of BAC331191 of human chromosome 19. CD20 was positive in the 60 DLBCL cases. Positive expression rates of CD10, Bclª²6 and MUM1 were 43.3%, 46.7% and 61.7% respectively. 32 (53.3%) were GCB and 28 (46.7%) were nonª²GCB. Bclª²2/IgH gene rearrangementª²positive cases were all GCB subtypes, which were significantly correlated with the expression of CD10 (P=0.012) and not with Bclª²6 and MUM1. CONCLUSION: Selfª²designed heminested PCR can improve the detection accuracy of bclª²2/IgH gene rearrangement. Bclª²2/IgH gene rearrangement detection helps the DLBCL determination of GCB subtypes. ......